Western blot was performed using Anti-LAT Monoclonal Antibody (LAT-01)(Product # MA1-19307) and a 34kDa, 28kDa, 27kDa and 24kDa band corresponding to LAT was observed across in T cell line, Jurkat, and Natural Killer cells, NK-92, but not in B-cells (Raji and Daudi). Membrane enriched extracts (30 µg lysate) of Jurkat (Lane 1), Raji (Lane 2), Daudi (Lane 3), NK-92 (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1250) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:8000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).Linker for T cell activation (LAT) is preferentially expressed in higher levels in T-cells and Natural Killer cells for its development and proliferation. Additional proteolytically cleaved products, reported at several molecular weights >13kDa, are detected in Jurkat and NK-cells in comparison to the B-cell lines tested. [doi:10.3389/fimmu.2018.00115, 10.1042/BJ20121135]